Bcl9 Knockout cell line(Hepa1-6)

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Bcl9 Knockout cell line(Hepa1-6) Order

Bcl9 Knockout cell line(Hepa1-6)
Catalog#
YKO-M087
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Bcl9 Knockout cell line(Hepa1-6)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Bcl9 Knockout cell line (Hepa1-6)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Mouse
Gene Name
Bcl9
Gene ID
77578
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 5 (based on transcript Bcl9-201)
Validation: PCR+Sanger Sequencing
Bcl9 Knockout cell line(Hepa1-6) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Hepa1-6
STR Info (Cell bank)
Cell Line:Hepa 1-6
Allele1Allele2Allele3Allele1Allele2Allele3
4-2 229
【18.3】
233.12
【19.3】
18.319.3
5-5 347.54
【17】
17.0
6-4 299.48
【18】
303.51
【18】
18.019.0
6-7 345.94
【15】
15.0
9-2 220.65
【15】
15.0
12-1 229.12
【17】
17.0
15-3 176.95
【17】
181.02
【18】
185.08
【19】
17.018.019.0
18-3 151.1
【16】
155.50
【17】
16.017.0
X-1 399.97
【25】
25.0
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Bcl9 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hepa1-6 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Bcl9 gene knockout Bcl9 Knockout cell line (Hepa1-6) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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